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EB09199 Goat Anti-SULF1 Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $300.00 Elite

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EB09199 1
EB09199 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: sulfatase FP, SULF-1, KIAA1077, HSULF-1, FLJ41750, FLJ38022, FLJ30905, sulfatase 1, SULF1
Official Symbol: SULF1
Accession Number(s): NP_055985.2
Human GeneID(s): 23213
Non-Human GeneID(s): 240725 (mouse), 171396 (rat)
Important Comments: Reported variants represent identical protein: NP_055985.2, NP_001121676.1, NP_001121678.1, NP_001121677.1.

Immunogen

Peptide with sequence C-QSYYNKEKGVKKQ, from the internal region of the protein sequence according to NP_055985.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 110kDa band observed in HumanTestis lysates (calculated MW of 101kDa according to NP_055985.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Background Reference

Yue X, Li X, Nguyen HT, Chin DR, Sullivan DE, Lasky JA.
Transforming growth factor-beta1 induces heparan sulfate 6-O-endosulfatase 1 expression in vitro and in vivo.
J Biol Chem. 2008 Jul 18;283(29):20397-407.
PMID: 18503048

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