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Goat Anti-Survivin / BIRC5 Antibody (EB06448)

Related Products: EBP06448 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06448 Goat Anti-Survivin / BIRC5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Cat, Dog, Pig, Cow $315.00 Elite

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EB06448 1
EB06448 (0.01µg/ml) staining of Molt-4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: survivin variant 3 alpha, baculoviral IAP repeat-containing protein 5, baculoviral IAP repeat-containing 5, apoptosis inhibitor 4, baculoviral IAP repeat-containing 5 (survivin), EPR-1, API4, survivin, BIRC5
Official Symbol: BIRC5
Accession Number(s): NP_001159.2; NP_001012271.1
Human GeneID(s): 332
Important Comments: This antibody is expected to recognize the reported isoforms 1 (NP_001159.2) and isoform 3 (NP_001012271.1).

Immunogen

Peptide with sequence CVRRAIEQLAAMD, from the C Terminus of the protein sequence according to NP_001159.2; NP_001012271.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 18kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4kDa according to NP_001159.2 and 18.6kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cat, Dog, Pig, Cow

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