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Goat Anti-Swiprosin 1 / EFHD2 Antibody (EB06552)

Related Products: EBP06552 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06552 Goat Anti-Swiprosin 1 / EFHD2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06552 1
EB06552 (0.5µg/ml) staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: EFHD2, MGC4342, EF hand domain containing 2
Official Symbol: EFHD2
Accession Number(s): NP_077305.2
Human GeneID(s): 79180
Non-Human GeneID(s): 27984 (mouse)
Important Comments: This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.

Immunogen

Peptide with sequence CAAFKELQSTFK, from the C Terminus of the protein sequence according to NP_077305.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 30kDa band observed in Human Thymus, Human Lymph node and Human Tonsil lysates (calculated MW of 26.7kDa according to NP_077305.2). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody (previous batch) has been successfully used in Western blot on Human:
Brachs S, Lang C, Buslei R, Purohit P, Fürnrohr B, Kalbacher H, Jäck HM, Mielenz D.
Monoclonal antibodies to discriminate the EF hand containing calcium binding adaptor proteins EFhd1 and EFhd2.
Monoclon Antib Immunodiagn Immunother. 2013 Aug;32(4):237-45.
PMID: 23909416

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