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Goat Anti-TANK2 Antibody (EB02504)

Code Name Applications Availability Size Tested species Price Grade  
EB02504 Goat Anti-TANK2 Antibody WB-Trf Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB02504 1
HEK293 overexpressing TANK2 (lane 4) and TANK1 (lane 2) and probed with EB02504 (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4°C. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TNKS2, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, tankyrase 2
Official Symbol: TNKS2
Accession Number(s): NP_079511.1
Human GeneID(s): 80351
Important Comments: Fusion protein containing 73 aas from region aa800-900 (according to NP_079511.1). No crossreactivity expected with TANK1.

Immunogen

Fusion protein containing 73 aas from aa800-900 region of the protein sequence according to NP_079511.1. No crossreactivity expected with TANK1.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Western blot: In transfected HEK293 transiently expressing TANK2 a band of approx. 127kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 127 kDa according to NP_079511.1. Recommended concentration, 0.5-1.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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