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Goat Anti-TCF2 / VHNF1 Antibody (EB07588)

Code Name Applications Availability Size Tested species Price Grade  
EB07588 Goat Anti-TCF2 / VHNF1 Antibody Pep-ELISA, WB, ICC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07588 1
EB07588 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TCF2, VHNF1, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, transcription factor 2
Official Symbol: HNF1B
Accession Number(s): NP_000449.1; NP_001159395.1
Human GeneID(s): 6928
Non-Human GeneID(s): 21410 (mouse), 25640 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000449.1; NP_001159395.1). This product may cross-react with HNF1A.

Immunogen

Peptide with sequence C-QAYDRQKNPSKEER, from the internal region of the protein sequence according to NP_000449.1; NP_001159395.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish

Specific Reference

This antibody has been successfully used in Western blot in Mouse:
Arenas F, Hervias I, Uriz M, Joplin R, Prieto J, Medina JF.
Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells.
J Clin Invest. 2008 Feb;118(2):695-709.
PMID: 18188457

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