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Code Name Applications Availability Size Tested species Price Grade  
EB11068 Goat Anti-thyroid peroxidase Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11068 1
EB11068 (5µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11068 2
EB11068 (0.1µg/ml) staining of Thyroid Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MSA, OTTHUMP00000115535, OTTHUMP00000115536, TDH2A, thyroid microsomal antigen, thyroid peroxidase, thyroperoxidase, TPX, TPO
Official Symbol: TPO
Accession Number(s): NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1
Human GeneID(s): 7173
Important Comments: This antibody is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1). Reported variants represent identical protein: NP_000538.3, NP_001193673.1 Reported variants represent identical protein: NP_783650.1, NP_001193674.1

Immunogen

Peptide with sequence ELRCGKHQAVGTSPQ, from the C Terminus of the protein sequence according to NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110kDa band observed in Human Thyroid Gland lysates (calculated MW of 103kDa according to NP_000538.3). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Thyroid Gland shows textured staining in the cytoplasm of epithelial cells. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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