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Goat Anti-TIA1 Antibody (EB07741)

Code Name Applications Availability Size Tested species Price Grade  
EB07741 Goat Anti-TIA1 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07741 1
EB07741 (0.1µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07741 2
EB07741 (3.75µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07741 3
EB07741 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: TIA1, TIA1 cytotoxic granule-associated RNA binding protein, TIA1 cytotoxic granule-associated RNA-binding protein, TIA1 protein, cytotoxic granule-associated RNA-binding protein, p40-TIA-1 (containing p15-TIA-1)
Official Symbol: TIA1
Accession Number(s): NP_071320.1; NP_071505.1
Human GeneID(s): 7072
Non-Human GeneID(s): 21841 (mouse), 312510 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).

Immunogen

Peptide with sequence C-PKSTYESNTKQ, from the internal region of the protein sequence according to NP_071320.1; NP_071505.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Thyroid. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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