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Goat Anti-TRIM5 / RNF88 Antibody (EB05932)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05932 | Goat Anti-TRIM5 / RNF88 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $315.00 |  |
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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: tripartite motif-containing protein 5 alpha, tripartite motif protein TRIM, OTTHUMP00000069812, tripartite motif protein TRIM5, tripartite motif protein 5, TRIM5alpha, tripartite motif-containing 5, RNF88, TRIM5Official Symbol: TRIM5
Accession Number(s): NP_149023.1; NP_149083.1; NP_149084.1
Human GeneID(s): 85363
Important Comments: This antibody is expected to recognise all three human isoforms of this protein.
Immunogen
Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1; NP_149083.1; NP_149084.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Approx 38kDa band observed in Human Plancenta and Human Uterus lysates (calculated MW of 37.7kDa according to NP_149084.1). Recommended concentration: 2-6µg/ml.
IHC: A previous batch was successfully used on Human Tonsil.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human
Specific References
The goat polyclonal antibody used in this paper was manufactured by us:
Yap MW, Lindemann D, Stanke N, Reh J, Westphal D, Hanenberg H, Ohkura S, Stoye JP.
Restriction of foamy viruses by primate Trim5alpha.
J Virol. 2008 Jun;82(11):5429-39.
PMID: 18367529
The goat polyclonal antibody used in this paper was manufactured by us:
Yap MW, Mortuza GB, Taylor IA, Stoye JP.
The design of artificial retroviral restriction factors.
Virology. 2007 Sep 1;365(2):302-14.
PMID: 17493656
The goat polyclonal antibody used in this paper was manufactured by us:
Ohkura S, Yap MW, Sheldon T, Stoye JP.
All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction.
J Virol. 2006 Sep;80(17):8554-65.
PMID: 16912305