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Goat Anti-UBID4 (aa245-259) Antibody (EB12124)

Related Products: EBP12123 - Immunizing Peptide, EBP12124 - Immunizing Peptide, Goat Anti-UBID4 (aa89-103) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12124 Goat Anti-UBID4 (aa245-259) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12124 1
EB12124 (1µg/ml) staining of Jurkat (A) and K562 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12124 2
EB12124 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DPF2, D4, zinc and double PHD fingers family 2, REQ, UBID4, ubi-d4, BAF45D, BRG1-associated factor 45D, apoptosis response zinc finger protein, protein requiem, requiem, apoptosis response zinc finger, zinc finger protein ubi-d4
Official Symbol: DPF2
Accession Number(s): NP_006259.1
Human GeneID(s): 5977
Non-Human GeneID(s): 19708 (mouse), 361711 (rat)

Immunogen

Peptide with sequence C-QPPTPVSQRSEEQKS, from the internal region of the protein sequence according to NP_006259.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 50kDa and 48kDa bands observed in lysates of cell lines Jurkat and K562 respectively (calculated MW of 44.2kDa according to NP_006259.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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