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Goat Anti-UBR2 Antibody (EB09658)

Related Products: EBP09658 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09658 Goat Anti-UBR2 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09658 1
EB09658 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBR2, ubiquitin protein ligase E3 component n-recognin 2, C6orf133, DKFZp686C08114, KIAA0349, MGC71112, RP3-392M17.3, bA49A4.1, dJ242G1.1, dJ392M17.3, OTTHUMP00000016403, OTTHUMP00000016405, OTTHUMP00000039767, OTTHUMP00000039768, ubiquitin ligase E3 alpha-II
Official Symbol: UBR2
Accession Number(s): NP_056070.1
Human GeneID(s): 23304
Non-Human GeneID(s): 224826 (mouse)

Immunogen

Peptide with sequence SCPKSGGDKSRAPT, from the internal region of the protein sequence according to NP_056070.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 150kDa in Rat Testis lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 199kDa according to Rat NP_001171542.1. The detected band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: Paraffin embedded Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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