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Goat Anti-Vimentin Antibody (EB11207)

Code Name Applications Availability Size Tested species Price Grade  
EB11207 Goat Anti-Vimentin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

Ordering Information for

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11207 1
EB11207 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11207 2
EB11207 (0.1µg/ml) staining of Mouse (A) and (2µg/ml) Rat (B) Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FLJ36605, vimentin, VIM
Official Symbol: VIM
Accession Number(s): NP_003371.2
Human GeneID(s): 7431
Non-Human GeneID(s): 22352 (mouse), 81818 (rat)

Immunogen

Peptide with sequence C-QVINETSQHHDDLE, from the C Terminus of the protein sequence according to NP_003371.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1). Recommended concentration: 0.1-2µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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