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Goat Anti-VPS11 Antibody (EB06828)

Code Name Applications Availability Size Tested species Price Grade  
EB06828 Goat Anti-VPS11 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB06828 1
EB06828 (3.8µg/ml) staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06828 2
EB06828 (4µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: VPS11, vacuolar protein sorting 11 (yeast), HGNC:14583, END1, PEP5, RNF108, hVPS11, vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting protein 11, vacuolar protein sorting 11 homolog (S. cerevisiae)
Official Symbol: VPS11
Accession Number(s): NP_068375.3
Human GeneID(s): 55823
Non-Human GeneID(s): 71732 (mouse)

Immunogen

Peptide with sequence C-HALQEKDTQTKLE, from the internal region of the protein sequence according to NP_068375.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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