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Goat Anti-VPS35 / MEM3 Antibody (EB06268)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06268 | Goat Anti-VPS35 / MEM3 Antibody | Pep-ELISA, WB, IHC, IF |  |
100µg specific antibody in 200µl | Human, Mouse, Rat, Cow | $315.00 |  |
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Target Protein
Principal Names: vacuolar protein sorting 35, vacuolar protein sorting 35 homolog (S. cerevisiae), maternal-embryonic 3, vacuolar protein sorting 35 (yeast), DKFZp434P1672, DKFZp434E1211, FLJ20388, FLJ13588, FLJ10752, MEM3, VPS35Official Symbol: VPS35
Accession Number(s): NP_060676.2
Human GeneID(s): 55737
Non-Human GeneID(s): 65114 (mouse)
Important Comments: Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Immunogen
Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 85kDa band observed in Human Brain Cerebellum lysates (predicted MW of 91kDa according to NP_060676.2). Recommended for use at 0.01-1µg/ml.
IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis. Data obtained with a previous batch.
Immunofluorescence: Staining of VPS35 (red) in human melanoma cells 1205LU. Please see Scott KL et al.; Nature. 2009 Jun 25;459(7250):1085-90.; PMID: 19553991. Data obtained with a previous batch.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Cow
Specific References
The goat polyclonal antibody used in this paper was manufactured by us:
Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L.
GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer.
Nature. 2009 Jun 25;459(7250):1085-90.
PMID: 19553991
The goat polyclonal antibody used in this paper was manufactured by us:
Gokool S, Tattersall D, Seaman MN.
EHD1 interacts with retromer to stabilize SNX1 tubules and facilitate endosome-to-Golgi retrieval.
Traffic. 2007 Dec;8(12):1873-86.
PMID: 17868075
The goat polyclonal antibody used in this paper was manufactured by us:
Nielsen MS, Gustafsen C, Madsen P, Nyengaard JR, Hermey G, Bakke O, Mari M, Schu P, Pohlmann R, Dennes A, Petersen CM.
Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA.
Mol Cell Biol. 2007 Oct;27(19):6842-51.
PMID: 17646382