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|EB06268||Goat Anti-VPS35 / MEM3 Antibody||Pep-ELISA, WB, IHC||100µg specific antibody in 200µl||Human, Rat||$315.00|
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Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunocytochemistry: Vesicular staining of VPS35 (green) in HEK293 and in SHSY5Y. Based on these data, a previous batch of this antibody was endorsed by PabmAbs (http://pabmabs.com/wordpress/?p=1523).
This antibody has been successfully used in IF on Human:
Takatori S, Tatematsu T, Cheng J, Matsumoto J, Akano T, Fujimoto T.
Phosphatidylinositol 3,5-Bisphosphate-Rich Membrane Domains in Endosomes and Lysosomes
Traffic. 2016 Feb;17(2):154-67
This antibody has been successfully used in ICC on CHO cells:
Lee S, Uchida Y, Emoto K, Umeda M, Kuge O, Taguchi T, Arai H.
Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis.
Genes Cells. 2012 Aug;17(8):728-36. doi: 10.1111/j.1365-2443.2012.01622.x.