Goat Anti-Smooth muscle alpha-actin Antibody

$464.00

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SKU: EB06450
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
smooth muscle alpha-actin|growth-inhibiting gene 4|alpha-cardiac actin|alpha 2 actin|ACTSA|AAT6|ACTSA|ACTA2|OTTHUMP00000020042|actin, alpha 2, smooth muscle, aorta
Accession ID
NP_001604.1; NP_001135417.1
Blocking Peptide
EBP06450
Immunogen
Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1; NP_001135417.1.
Product Comments
Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Peptide Sequence
EEEDSTALVC
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Mouse, Rat
Human Gene ID
59
Product Grade
IHC Results
S4C4P26
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 42kDa band observed in Human Duodenum lysates, Rat Heart lysates, and in lysates of cell lines HeLa and NIH3T3 (calculated MW of 42kDa according to Human NP_001604, Mouse NP_031418.1 and Rat NP_112266.1) . Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 22912365, "intro": "This antibody (previous batch) has been successfully used in IHC on Mouse:", "title": "Exosomal secretion of death bullets: a new way of apoptotic escape?", "author": "Trokovic N, Pöllänen R, Porola P, Stegaev V, Hetzel U, Tivesten Å, Engdahl C, Carlsten H, Forsblad-d'Elia H, Fagman JB, Lagerquist M, Konttinen YT.", "journal": "Am J Physiol Endocrinol Metab. 2012 Oct 15;303(8):E1015-24."}, {"pmid": 23383100, "intro": "This antibody (previous batch) has been successfully used in IHC on Mouse:", "title": "Modification of hemodynamic and immune responses to exposure with a weak antigen by the\r\nexpression of a hypomorphic BMPR2 gene.", "author": "Park SH, Chen WC, Hoffman C, Marsh LM, West J, Grunig G.", "journal": "PLoS One. 2013;8(1):e55180."}, {"pmid": 18538388, "intro": "This antibody (previous batch) has been successfully used in IF on Human:", "title": "Novel HLA-G-binding leukocyte immunoglobulin-like receptor (LILR) expression patterns in human placentas and umbilical cords.", "author": "McIntire RH, Sifers T, Platt JS, Ganacias KG, Langat DK, Hunt JS.", "journal": "Placenta. 2008 Jul;29(7):631-8."}, {"pmid": 25726846, "intro": "This antibody (previous batch) has been successfully used in IHC on Human:", "title": "High-mobility group box-1 induces vascular remodelling processes via c-Jun activation.", "author": "Zabini D, Crnkovic S, Xu H, Tscherner M, Ghanim B, Klepetko W, Olschewski A, Kwapiszewska G, Marsh LM.", "journal": "J Cell Mol Med. 2015 May;19(5):1151-61"}, {"pmid": 27704164, "intro": "This antibody (previous batch) has been successfully used in IHC on Mouse:", "title": "Human multipotent adult progenitor cells enhance islet function and revascularisation when co-transplanted as a composite pellet in a mouse model of diabetes.", "author": "João Paulo M. C. M. Cunha, Gunter Leuckx, Peter Sterkendries, Hannelie Korf, Gabriela Bomfim-Ferreira, Lutgart Overbergh, Bart Vaes, Harry Heimberg, Conny Gysemans, Chantal Mathieu", "journal": "Diabetologia. 2016 Oct 4."}, {"pmid": 26349856, "intro": "This antibody (previous batch) has been successfully used in WB and IF on Mouse:", "title": "Topical Application of Insulin Accelerates Vessel Maturation of Wounds by Regulating Angiopoietin-1 in Diabetic Mice.", "author": "Chaofei Li, Tianyi Yu, Yan Liu, Xuelian Chen, and Xiong Zhang.", "journal": "Int J Low Extrem Wounds. 2015 Dec;14(4):353-64. "}, {"pmid": 25034560, "intro": "This antibody (previous batch) has been successfully used in IHC on Human:", "title": "Comprehensive analysis of inflammatory markers in chronic thromboembolic pulmonary hypertension patients.", "author": "Diana Zabini, Akos Heinemann, Vasile Foris, Chandran Nagaraj, Patrick Nierlich, Zolta´n Ba´lint, Grazyna Kwapiszewska, Irene M. Lang, Walter Klepetko, Horst Olschewski and Andrea Olschewski.", "journal": "Eur Respir J. 2014 Oct;44(4):951-62. "}, {"pmid": 28705908, "intro": "This antibody (previous batch) has been successfully used in ICC on Human:", "title": "Importance of kynurenine in pulmonary hypertension", "author": "Nagy BM, Nagaraj C, Meinitzer A, Sharma N, Papp R, Foris V, Ghanim B, Kwapiszewska G, Kovacs G, Klepetko W, Pieber TR, Mangge H, Olschewski H, Olschewski A", "journal": "Am J Physiol Lung Cell Mol Physiol. 2017 Nov 1;313(5):L741-L751."}, {"pmid": 34309864, "intro": "This antibody (previous batch) has been successfully used in IHC on Mouse:", "title": "Low oxygen levels decrease adaptive immune responses and ameliorate experimental asthma in mice", "author": "Mathias Hochgerner, Eva M Sturm, Diana Schnoegl, Grazyna Kwapiszewska, Horst Olschewski, Leigh M Marsh", "journal": "Allergy. 2021 Jul 26. doi: 10.1111/all.15020"}]