Goat Anti-ADAMTSL1 / Punctin (isoform 2) Antibody

$255.00

Category: Primary Antibodies
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SKU: EB05359
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ADAM-TS related protein 1|thrombospondin|punctin|MGC40193|ADAMTSR1|ADAMTS-like 1|ADAMTSL1
Accession ID
NP_443098
Immunogen
Peptide with sequence CQELEEGAAVSEEPS, from the C Terminus of the protein sequence according to NP_443098.
Product Comments
This antibody is expected to recognise isoform 2 (NP_443098; also known as punctin), not human isoforms 1 and 3 (NP_640329 and NP_644669).
Peptide Sequence
CQELEEGAAVSEEPS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Human Gene ID
92949
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Western Blot: Approx. 50-55kDa band observed in Human Heart lysate at 0.5ug of primary. Please note that the band we observe is different from the predicted size of approx. 60kDa according to NP_443098. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
Application Type
Pep-ELISA

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