Goat Anti-VPS33B (internal) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

vacuolar protein sorting 33B (yeast homolog)|vacuolar protein sorting 33B (yeast homolog)|FLJ14848|HGNC:12712|vacuolar protein sorting 33B (yeast)|VPS33B

Immunohistochemistry: Preliminary experiments in Human Kidney showed staining of epithelial cells of renal glomeruli and some renal tubules. Recommended concentration 5-10µg.

NP_061138.2

Peptide with sequence CESIMKKKTKQDFQ, from the internal region of the protein sequence according to NP_061138.2.

CESIMKKKTKQDFQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Yeast

26276

Antibody detection limit dilution 1:32000.

Western Blot: Preliminary experiments gave an approx 48kDa band in Human lysates of kidney fibroblast cell line HEK293 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.6kDa according to NP_061138.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA