Goat Anti-EHD4 Antibody

$255.00

Category: Primary Antibodies
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SKU: EB06855
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
hepatocellular carcinoma-associated protein HCA11|ortholog of rat pincher|EH domain containing 4|PAST4|HGNC:3245|EH-domain containing 4|EHD4
Accession ID
NP_644670.1
Blocking Peptide
EBP06855
Immunogen
Peptide with sequence C-SHRKSLPKAD, from the C Terminus of the protein sequence according to NP_644670.1.
Peptide Sequence
C-SHRKSLPKAD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Human Gene ID
30844
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 28-30kDa band in human breast and testes lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.1kDa according to NP_644670.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA

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