Goat Anti-CAPON / NOS1AP (internal) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

ligand of neuronal nitric oxide synthase with carboxyl-terminal PDZ domain|C-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON)|C-terminal PDZ domain ligand of neuronal nitric oxide synthase|6330408P19Rik|CAPON|nitric oxide synthase 1 (neuronal) adaptor protein|NOS1AP

NP_055512.1

EBP07119

Peptide with sequence CNVFKSKKKSQ, from the internal region of the protein sequence according to NP_055512.1.

CNVFKSKKKSQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

9722

70729

192363

Antibody detection limit dilution 1:8000.

Western Blot: Preliminary experiments gave bands at approx 50kDa and 35kDa in Human Brain (Frontal Cortex) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 56.1kDa according to NP_055512.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA