Goat Anti-DAGLB Antibody

$255.00

Category: Primary Antibodies
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SKU: EB08791
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
diacylglycerol lipase beta|KCCR13L|FLJ33909|FLJ33624|DAGLBETA|diacylglycerol lipase, beta|DAGLB
Accession ID
NP_631918.1; NP_001136408.1
Immunogen
Peptide with sequence C-KWSHEAEFSKIL, from the internal region of the protein sequence according to NP_631918.1; NP_001136408.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_631918.1; NP_001136408.1).
Peptide Sequence
C-KWSHEAEFSKIL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Human Gene ID
221955
Mouse Gene ID
231871
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Preliminary experiments gave an approx 50kDa band in Human Brain (Cerebellum, Amygdala and Substantia nigra) lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 73.7kDa according to NP_631918.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA

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