Goat Anti-SGLT1 Antibody

$423.00

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SKU: EB09310
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
solute carrier family 5 (sodium/glucose transporter), member 1|sodium/glucose cotransporter 1|Human Na+/glucose cotransporter 1 mRNA, complete cds|SGLT1|NAGT|D22S675|solute carrier family 5 (sodium/glucose cotransporter), member 1|SLC5A1
Accession ID
NP_000334.1; NP_001243243.1
Blocking Peptide
EBP09310
Immunogen
Peptide with sequence C-KETIEIETQVPEKKK, from the internal region of the protein sequence according to NP_000334.1; NP_001243243.1.
Product Comments
This antibody is expected to recognize isoforms 1 ((NP_000334.1) and isoform 2 (NP_001243243.1).
Peptide Sequence
C-KETIEIETQVPEKKK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
6523
Product Grade
IHC Results
Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed a band at approx. 60kDa in Human Gall Bladder lysate and in Jurkat cell lysate after 1-3µg/ml antibody staining (calculated MW of 60.1kDa according to NP_001243243.1). Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IHC
Selected References
[{"pmid": 34198013, "intro": "This antibody (previous batch) has been successfully used in Western blot and IHC on Mouse:", "title": "Aberrant Epithelial Differentiation Contributes to Pathogenesis in a Murine Model of Congenital Tufting Enteropathy.", "author": "Barun Das, Kevin Okamoto, John Rabalais, Jocelyn A. Young, Kim E. Barrett, and Mamata Sivagnanam", "journal": "Cell Mol Gastroenterol Hepatol. 2021; 12(4): 1353-1371."}, {"pmid": 28174043, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "Sodium influx through cerebral sodium-glucose transporter type 1 exacerbates the development of cerebral ischemic neuronal damage.", "author": "Yamazaki Y, Harada S, Wada T, Hagiwara T, Yoshida S, Tokuyama S", "journal": "Eur J Pharmacol. 2017 Mar 15;799:103-110."}, {"pmid": 30503674, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "Activation of c-Jun N-terminal kinase and p38 after cerebral ischemia upregulates cerebral sodium-glucose transporter type 1", "author": "Yui Yamazaki, Kyoko Arita, Shinichi Harada, Shogo Tokuyama", "journal": "Journal of Pharmacological Sciences (November 2018)"}]