Goat Anti-POFUT1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB11528
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
protein O-fucosyltransferase 1|POFUT1|peptide-O-fucosyltransferase 1|OTTHUMP00000030584|OTTHUMP00000030583|O-FUT|O-FucT-1|O-Fuc-T|o-fucosyltransferase protein|MGC2482|KIAA0180|GDP-fucose protein O-fucosyltransferase 1|FUT12
Usage Summary
Purchase the +ve control! The transfected lysate used in QC is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
Accession ID
NP_056167.1; NP_758436.1
Blocking Peptide
EBP11528
Immunogen
Peptide with sequence C-TNLHVSYQKYFKLEP, from the internal region of the protein sequence according to NP_056167.1; NP_758436.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_056167.1; NP_758436.1).
Peptide Sequence
C-TNLHVSYQKYFKLEP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
23509
Mouse Gene ID
140484
Rat Gene ID
311551
Product Grade
IHC Results
In paraffin embedded Human Cerebral Cortex shows staining of the cytoplasm in neuronal bodies. Recommended concentration, 2-4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
In transfected HEK293 transiently expressing Human POFUT1 (myc and DYKDDDDK tagged), a band of approx. 48kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.3-1µg/ml.
Application Type
Pep-ELISA, WB-Trf, IHC

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