Goat Anti-Ubinuclein 1 Antibody

$255.00

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SKU: EB11599
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
VT4|VT|UBN1|ubiquitously expressed nuclear protein|ubinuclein-1|ubinuclein 1|OTTHUMP00000159926|OTTHUMP00000159925|HIRA-binding protein
Accession ID
NP_058632.2
Blocking Peptide
EBP11599
Immunogen
Peptide with sequence C-SQLHGKGPAVPRK, from the C Terminus of the protein sequence according to NP_058632.2.
Product Comments
Reported variants represent identical protein: NP_058632.2, NP_001072982.1.
Peptide Sequence
C-SQLHGKGPAVPRK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Human Gene ID
29855
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary experiments gave an approx 100kDa band in HeLa lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 122kDa according to NP_058632.2. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA