Goat Anti-CD163 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB12340
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
scavenger receptor cysteine-rich type 1 protein M130|macrophage-associated antigen|hemoglobin scavenger receptor|MM130|M130|CD163 molecule|CD163
Usage Summary
Flow Cytometry: Flow cytometric analysis of Caco-2 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_004235.4; NP_981961.2;
Blocking Peptide
EBP12340
Immunogen
Peptide with sequence C-SRGENLVHQIQYRE, from the internal region (near C terminus) of the protein sequence according to NP_004235.4; NP_981961.2;.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_004235.4; NP_981961.2).
Peptide Sequence
C-SRGENLVHQIQYRE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
9332
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx. 150kDa band observed in lysates of cell line Caco-2 (calculated MW of 125kDa according to NP_004235.4). Molecular weights of 150-200kDa have been observed by other commercial sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC

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