Goat Anti-UBE2C / UBCH10 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05569
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
RP3-447F3.1|mitotic-specific ubiquitin-conjugating enzyme|cyclin-selective ubiquitin carrier protein|ubiquitin carrier protein E2-C|ubiquitin-protein ligase C|ubiquitin-conjugating enzyme E2C|dJ447F3.2|UBCH10|UBE2C
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.

Accession ID
NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1; NP_001268670.1
Blocking Peptide
EBP05569
Immunogen
Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1; NP_001268670.1.
Product Comments
This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Peptide Sequence
C-QETYSKQVTSQEP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
11065
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC, IP

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