Goat Anti-GATA1 Antibody

$464.00

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SKU: EB05571
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ERYF1|NFE1|GF1|GATA binding protein 1 (globin transcription factor 1)|GATA1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002040.1
Blocking Peptide
EBP05571
Immunogen
Peptide with sequence C-PTTSTTVVAPLSS, from the C Terminus of the protein sequence according to NP_002040.1.
Peptide Sequence
C-PTTSTTVVAPLSS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
2623
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 50kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Hippocampus lysate.
Application Type
Pep-ELISA, WB, IF, FC