Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names pEg3 kinase|OTTHUMP00000046113|HPK38|RP11-8N6.1|KIAA0175|maternal embryonic leucine zipper kinase|MELK | |
Accession ID NP_055606.1 | |
Blocking Peptide EBP05041 | |
Immunogen Peptide with sequence KRLVEDILSSCKV, from the C Terminus of the protein sequence according to NP_055606.1. | |
Peptide Sequence KRLVEDILSSCKV | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Dog, Cow | |
Reactive Species Human | |
Human Gene ID 9833 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Experiments gave bands at approx 75kDa and 150kDa in Human Testis lysates and in cell lines HeLa and Jurkat after 0.1µg/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3µg/ml. Preliminary testing was unsuccessful on Mouse Testis for this particular batch. | |
Application Type Pep-ELISA, WB |
Goat Anti-MELK Antibody
$423.00
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