Goat Anti-BIF-1 / SH3GLB1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05158
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
dJ612B15.2|SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1|SH3-domain, GRB2-like, endophilin B1|SH3-containing protein SH3GLB1|Bax-interacting factor 1|endophilin B1|KIAA0491|CGI-61|SH3-domain GRB2-like endophilin B1|Bif-1|SH3GLB1
Usage Summary
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_057093.1; NP_001193580.1; NP_001193581.1; NP_001193582.1
Blocking Peptide
EBP05158
Immunogen
Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1; NP_001193580.1; NP_001193581.1; NP_001193582.1.
Peptide Sequence
C-QKGKVPITYLELLN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
51100
Mouse Gene ID
54673
Product Grade
IHC Results
Paraffin embedded Human Colon. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 40kDa band observed in lysates of cell lines HEK293 and NIH3T3, and approx. 38kDa in lysates of cell lines A431, RAW264.7 and KNRK (calculated MW of 40.8kDa according to Human NP_057093.1, Mouse NP_062337.1 and Rat NP_001011929.1). Recommended concentration:0.1-0.3µg/ml. Primary incubated 1 hour.at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC

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