Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names CMG1\|coiled-coil domain containing 2\|capillary morphogenesis protein 1\|intraflagellar transport 74 homolog (Chlamydomonas)\|MGC111562\|FLJ22621\|CMG-1\|CCDC2\|IFT74
Accession ID NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1
Blocking Peptide EBP06339
Immunogen Peptide with sequence C-KTIVDALHSTSGN, from the C Terminus of the protein sequence according to NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1.
Peptide Sequence C-KTIVDALHSTSGN
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Rat
Reactive Species Human
Human Gene ID 80173
Rat Gene ID 313365
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:32000.
Western Blot Approx 70kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276). Recommended concentration: 0.2-0.6µg/ml.
Application Type Pep-ELISA, WB

Selected References

[{"pmid": 17166276, "intro": "This antibody (previous batch) has been successfully used in IF on Rat:", "title": "Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.", "author": "Momeni P, Schymick J, Jain S, Cookson MR, Cairns NJ, Greggio E, Greenway MJ, Berger S, Pickering-Brown S, Chiò A, Fung HC, Holtzman DM, Huey ED, Wassermann EM, Adamson J, Hutton ML, Rogaeva E, St George-Hyslop P, Rothstein JD, Hardiman O, Grafman J, Singleton A, Hardy J, Traynor BJ.", "journal": "BMC Neurol. 2006 Dec 13;6:44"}]

Goat Anti-CMG1 / CCDC2 / IFT74 Antibody

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