Goat Anti-PLRG1 Antibody

$423.00

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SKU: EB05248
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
pleiotropic regulator 1 (PRL1, Arabidopsis homolog)|pleiotropic regulator 1 (PRL1homolog, Arabidopsis)|pleiotropic regulator 1 (PRL1, Arabidopsis homolog)|MGC110980|PRL1|pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)|PLRG1
Accession ID
NP_002660.1; NP_001188493.1
Blocking Peptide
EBP05248
Immunogen
Peptide with sequence C-PVSWKPEIIKRKRF, from the C Terminus of the protein sequence according to NP_002660.1; NP_001188493.1.
Product Comments
This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1).
Peptide Sequence
C-PVSWKPEIIKRKRF
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse
Human Gene ID
5356
Rat Gene ID
60376
Product Grade
IHC Results
Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 60kDa band observed in nuclear lysates of cell lines HEK293 and NIH3T3 (calculated MW of 57.2kDa according to Human NP_002660.1 and 56.9kDa according to Mouse NP_058064.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 20467437, "intro": "This antibody (previous batch) has been successfully used in WB and ICC on Human:", "title": "Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.", "author": "Llères D, Denegri M, Biggiogera M, Ajuh P, Lamond AI.", "journal": "EMBO Rep. 2010 Jun;11(6):445-51."}]