Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names nitric oxide synthase 1, neuronal|nNOS|IHPS1|penile neuronal NOS|penile neuronal nitric oxide synthase|PnNOS|neuronal nitric oxide synthase|NOS|nitric oxide synthase 1 (neuronal)|NOS1 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_000611.1; NP_001191147.1; NP_001191142.1 | |
Blocking Peptide EBP05259 | |
Immunogen Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1; NP_001191147.1; NP_001191142.1. | |
Peptide Sequence C-ESKKDTDEVFSS | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 4842 | |
Product Grade  | |
IHC Results Cryosections of Human Hypothalamus. Recommended dilution range: 1:10000 - 1:40000. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx. 160kDa band observed in Mouse Brain lysates (calculated MW of 160.5kDa according to NP_032738.1). Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IHC, IF, FC |
Goat Anti-NOS1 Antibody
$464.00
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Selected References [{"pmid": 20140458, "intro": "This antibody (previous batch) has been successfully used in IF on Mouse:", "title": "Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice.", "author": "Fried HU, Kaupp UB, Müller F.", "journal": "Cell Tissue Res. 2010 Mar;339(3):463-79."}] |