Goat Anti-Sak / STK18 / PLK4 Antibody

$423.00

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SKU: EB05336
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
STK 18|MCCRP2|serine/threonine protein kinase SAK|STK18|protein-serine/threonine kinase|polo-like kinase 4|Snk Akin Kinase|SAK|serine/threonine kinase 18|polo-like kinase 4 (Drosophila)|Sak|PLK4
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_055079.3, NP_001177728.1
Blocking Peptide
EBP05336
Immunogen
Peptide with sequence ATCIGEKIEDFKVGN, from the N Terminus of the protein sequence according to NP_055079.3, NP_001177728.1.
Peptide Sequence
ATCIGEKIEDFKVGN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Cow
Reactive Species
Human
Human Gene ID
10733
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 110kDa band observed in Human Colon lysates (calculated MW of 109kDa according to NP_055079.3). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IF, FC