Goat Anti-VAV2 Antibody

$423.00

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SKU: EB05337
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
VAV2|vav 2 oncogene|Oncogene VAV2|vav 2 guanine nucleotide exchange factor|Protein vav-2|oncogene VAV2
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_001127870.1; NP_003362.2
Blocking Peptide
EBP05337
Immunogen
Peptide with sequence ETEAKYYRTLEDIEC, from the internal region of the protein sequence according to NP_001127870.1; NP_003362.2.
Product Comments
This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).
Peptide Sequence
ETEAKYYRTLEDIEC
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig
Reactive Species
Human
Human Gene ID
7410
Mouse Gene ID
22325
Product Grade
IHC Results
Paraffin embedded Human Colon. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 100kDa band observed in 293 lysate (predicted MW of 100kDa according to NP_003362). Recommended for use at 1-1.5µg/ml.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]