Goat Anti-ARHU / WRCH1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

RHOU|ras homolog gene family, member U|ARHU|WRCH1|hG28K|WRCH-1|CDC42L1|FLJ10616|DJ646B12.2|fJ646B12.2|2310026M05Rik|ras homolog gene family, member U|Ryu GTPase|CDC42-like GTPase|GTP-binding protein like 1|Wnt-1 responsive Cdc42 homolog|FLJ10616|WRCH1|GTP-binding protein SB128|GTP-binding protein like 1|ras-like gene family member U

NP_067028.1

EBP05375

Peptide with sequence PPQQGDPAFPDRCEA, from the N Terminus of the protein sequence according to NP_067028.1.

PPQQGDPAFPDRCEA

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

58480

Antibody detection limit dilution 1:16000.

Preliminary experiments gave an approx. 22kDa band in lysates of cell lines A431, HeLa and HEK293 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.2kDa according to NP_067028.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA