Goat Anti-AXIN1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

AXIN1|axin 1|AXIN|LA16c-314G4.3|MGC52315|axis inhibition protein 1|axis inhibitor 1|fused, mouse, homolog of

NP_003493.1; NP_851393.1

EBP05380

Peptide with sequence C-FEEKIIGKVEKVD, from the C Terminus of the protein sequence according to NP_003493.1; NP_851393.1.

This antibody is expected to recognise both reported isoforms.

C-FEEKIIGKVEKVD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human

8312

12005

79257

In paraffin embedded Human Adrenal Gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5µg/ml.

Antibody detection limit dilution 1:128000.

Preliminary experiments gave bands at approx 150kDa and 22-24kDa in Human Brain (Hippocampus) lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6kDa according to NP_003493.1 and 91.7kDa according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Pep-ELISA, IHC