Goat Anti-MRGX Antibody

$423.00

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SKU: EB05410
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000023756|MSL3-2 protein|MORFL2|RP5-1055C14.2|mortality factor 4 like 2|KIAA0026 gene product|KIAA0026|MORF-related gene X|MRGX|MORF4L2
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_036418.1
Blocking Peptide
EBP05410
Immunogen
Peptide with sequence SSRKQGSQPRGQQS-C, from the N Terminus of the protein sequence according to NP_036418.1.
Peptide Sequence
SSRKQGSQPRGQQS-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
9643
Mouse Gene ID
56397
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 35kDa band observed in lysates of cell line A431 (calculated MW of 32.3kDa according to NP_036418.1). In transfected HEK293 transiently expressing MORF4L2 a band of approx. 37kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml. Primary incubation was for 1 hour.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]