Goat Anti-DAP3 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05427
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
mitochondrial 28S ribosomal protein S29|death-associated protein 3|bMRP-10|MRPS29|MRP-S29|MGC126059|MGC126058|DKFZp686G12159|DAP-3|death associated protein 3|DAP3
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_387506.1; NP_001186779.1; NP_001186780.1
Blocking Peptide
EBP05427
Immunogen
Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1; NP_001186779.1; NP_001186780.1.
Product Comments
This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).
Peptide Sequence
NPSLLERHCAYL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
7818
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 40kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41kDa according to NP_001186779.1 and NP_001186780). Recommended concentration: 0.1-1µg/ml. Primary incubated 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC

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