Goat Anti-ARPC2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05433
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
actin related protein 2/3 complex subunit 2|ARP2/3 protein compex subunit 34|PNAS-139|p34-Arc|PRO2446|ARC34|actin related protein 2/3 complex, subunit 2, 34kDa|ARPC2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.
Accession ID
NP_005722.1; NP_690601.1
Blocking Peptide
EBP05433
Immunogen
Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1; NP_690601.1.
Product Comments
The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
Peptide Sequence
C-EMKTITGKTFSSR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
10109
Mouse Gene ID
76709
Product Grade
IHC Results
In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 34-35kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3kDa according to NP_005722.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, IHC, WB

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