Goat Anti-SAP130 / SF3B3 Antibody

$423.00

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SKU: EB05543
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SF3b130|splicing factor 3b, subunit 3|spliceosome-associated protein 130|pre-mRNA splicing factor SF3b, 130 kDa subunit|STAF130|KIAA0017|SF3B130|RSE1|splicing factor 3b, subunit 3, 130kDa|SAP130|SF3B3
Accession ID
NP_036558.3
Blocking Peptide
EBP05543
Immunogen
Peptide with sequence C-VSKKLEDIRTRYAF, from the C Terminus of the protein sequence according to NP_036558.3.
Peptide Sequence
C-VSKKLEDIRTRYAF
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
23450
Mouse Gene ID
101943
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3µg/ml. Preliminary testing was unsuccessful on NIH3T3 for this particular batch. Primary incubated for 1 hour.
Application Type
Pep-ELISA, WB