Goat Anti-GCIP / MAID Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

D-type cyclin-interacting protein 1|grap2 cyclin interacting protein|MAID protein|HHM Protein|GCIP|DIP1|cyclin D-type binding-protein 1|CCNDBP1

NP_036274.3; NP_411241.1

EBP05706

Peptide with sequence C-NRIKELTQSELEL, from the C Terminus of the protein sequence according to NP_036274.3; NP_411241.1.

This antibody is expected to recognise both human isoforms of this protein.

C-NRIKELTQSELEL

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Dog

Human

23582

In paraffin embedded Human Prostate shows textured cytoplasm staining with emphasis on the luminal side of the secretory cells in the glands. Recommended concentration, 3-6µg/ml.

Antibody detection limit dilution 1:32000.

Preliminary experiments gave an approx 55kDa band in K562 lysate at 0.5ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 41kDda and 27kDa according to NP_036274; NP_411241. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC