Goat Anti-CDYL Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

DKFZp586C1622|testis-specific chromodomain Y-like protein|chromodomain protein, Y chromosome-like, isoform b|chromodomain protein, Y chromosome-like|bA620A17.2 (chromodomain protein, Y chromosome-like)|CDY-like, autosomal|CDYL1, DKFZP586C1622, MGC131936|chromodomain protein, Y-like|CDYL

NP_004815.3; NP_001137442.1; NP_001137443.1

EBP05779

Peptide with sequence C-SMLKYLQRKIDEF, from the C Terminus of the protein sequence according to NP_004815.3; NP_001137442.1; NP_001137443.1.

This antibody is expected to recognize both eported isoforms NP_004815.3; NP_001137442.1; NP_001137443.1). Variants (NP_001137442.1; NP_001137443.1) encode the same isoform.

C-SMLKYLQRKIDEF

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human

9425

12593

Antibody detection limit dilution 1:4000.

Approx 75kDa band observed in Human Testes lysates (calculated MW of 66.5kDa according to NP_004815.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8707-12; PMID: 12072557). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, WB