Goat Anti-KAP1 / TRIM28 Antibody

$464.00

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SKU: EB05810
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
tripartite motif-containing 28 protein|KRAB-associated protein 1|FLJ29029|transcriptional intermediary factor 1-beta|nuclear corepressor KAP-1|KRAB-associated protein 1|tripartite motif-containing 28|TIF1B|RNF96|TF1B|KAP1|TRIM28
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_005753.1
Blocking Peptide
EBP05810
Immunogen
Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.
Peptide Sequence
C-SSQELSGGPGDGP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
10155
Product Grade
IHC Results
In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Application Type
Pep-ELISA, IF, FC, IHC
Selected References
[{"pmid": 19898899, "intro": "This antibody (previous batch) has been successfully used in IHC on Human:", "title": "KAP1 Is Associated With Peritoneal Carcinomatosis in Gastric Cancer.", "author": "Yokoe T, Toiyama Y, Okugawa Y, Tanaka K, Ohi M, Inoue Y, Mohri Y, Miki C, Kusunoki M.", "journal": "Ann Surg Oncol. 2010 Mar;17(3):821-8."}]