Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1\|FLJ93118\|FLJ20329\|DKFZp686O13120\|ariadne, Drosophila, homolog of\|ariadne homolog\|ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila)\|UBCH7BP\|HARI\|ARI\|HHARI\|ARIH1
Accession ID NP_005735.2
Blocking Peptide EBP05812
Immunogen Peptide with sequence C-HEGYEKDLWEYIED, from the C Terminus of the protein sequence according to NP_005735.2.
Peptide Sequence C-HEGYEKDLWEYIED
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Cow
Reactive Species Human
Human Gene ID 25820
Mouse Gene ID 23806
Product Grade
IHC Results Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.
ELISA Detection Limit Antibody detection limit dilution 1:32000.
Western Blot Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1µg/ml. Primary incubated for 1 hour.
Application Type Pep-ELISA, WB, IHC

Selected References

[{"pmid": 33879767, "intro": "This antibody has been successfully used in the following paper:", "title": "ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation.", "author": "Youqian Wu, Chao Zhang, Xiaolan Liu, Zhengfu He, Bing Shan, Qingxin Zeng, Qingwei Zhao, Huaying Zhu, Hongwei Liao, Xufeng Cen, Xiaoyan Xu, Mengmeng Zhang, Tingjun Hou, Zhe Wang, Huanhuan Yan, Shuying Yang, Yaqin Sun, Yanying Chen, Ronghai Wu, Tingxue Xie, Wei Chen, Ayaz Najafov, Songmin Ying, and Hongguang Xia", "journal": "Nat Commun. 2021; 12: 2346."}, {"pmid": 37429863, "intro": "This antibody has been successfully used in IHC on Mouse:", "title": "ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade.", "author": "Xiaolan Liu, Xufeng Cen, Ronghai Wu, Ziyan Chen, Yanqi Xie, Fengqi Wang, Bing Shan, Linghui Zeng, J Zhou, Bojian Xie, Yangjun Cai, Jin-Yan Huang, Yingjiqiong Liang, Youqian Wu, Chao Zhang, Dongrui Wang, Hong-Guang Xia", "journal": "Nat Commun. 2023 Jul 10;14(1):4066."}]

Goat Anti-ARIH1 / HHARI Antibody

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