Goat Anti-FOXA1 / HNF3A Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05999
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
hepatocyte nuclear factor 3, alpha|MGC33105|TCF3A|forkhead box A1|HNF3A|FOXA1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_004487.2
Blocking Peptide
EBP05999
Immunogen
Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
Peptide Sequence
C-GVYSRPVLNTS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig, Cow
Reactive Species
Human, Mouse
Human Gene ID
3169
Mouse Gene ID
15375
Rat Gene ID
25098
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 50kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1kDa according to Human NP_004487.2 and 48.9kDa according to Mouse NP_032285.2).These bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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