Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names ERP1|early mitotic inhibitor 2|Fbx43|F-box protein 43|EMI2|FBXO43 | |
Accession ID NP_001025031.2 | |
Blocking Peptide EBP06061 | |
Immunogen Peptide with sequence C-PGSAQSKRNLKRL, from the C Terminus of the protein sequence according to NP_001025031.2. | |
Peptide Sequence C-PGSAQSKRNLKRL | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Mouse | |
Human Gene ID 286151 | |
Mouse Gene ID 78803 | |
Rat Gene ID 315034 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 80kDa band observed in Mouse Ovary lysates (calculated MW of 78.4kDa according to NP_001025031.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human, Rat and Pig Testis, and Human Ovary for this particular batch. | |
Application Type Pep-ELISA, WB |
Goat Anti-EMI2 Antibody
$423.00
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Selected References [{"pmid": 22302686, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "A zinc-dependent mechanism regulates meiotic progression in mammalian oocytes.", "author": "Bernhardt ML, Kong BY, Kim AM, O'Halloran TV, Woodruff TK.", "journal": "Biol Reprod. 2012 Apr 19;86(4):114."}, {"pmid": 27605379, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "The BTG4 and CAF1 complex prevents the spontaneous activation of eggs by deadenylating maternal mRNAs.", "author": "Pasternak M, Pfender S, Santhanam B, Schuh M", "journal": "Open Biol. 2016 Sep;6(9)"}, {"pmid": 19802389, "intro": "This antibody (previous batch) has been successfully used in Western blot, Immunocytochemistry and Flow Cytometry on Rat:", "title": "Cytostatic Factor Proteins Are Present in Male Meiotic Cells and ?-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes.", "author": "Perrard MH, Chassaing E, Montillet G, Sabido O, Durand P.", "journal": " PLoS One. 2009 Oct 5;4(10):e7237."}] |