Goat Anti-ORP5 (OBPH1) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

oxysterol-binding protein-like protein 5|FLJ42929|oxysterol-binding protein-related protein 5|oxysterol-binding protein homologue 1|OSBP-related protein 5|oxysterol binding protein-like 5|DKFZp586H1719|KIAA1534|FLJ31948|OBPH1|ORP5|OSBPL5

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_065947.1; NP_663613.1; NP_001137535.1

EBP06154

Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1; NP_663613.1; NP_001137535.1.

This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.

KEEAFLRRRFSLC

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

Human, Rat

114879

79196

Paraffin embedded Human Kidney and Liver. Recommended concentration: 5µg/ml.

Antibody detection limit dilution 1:32000.

Approx. 100kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4kDa according to Human NP_065947.1 and 101.7kDa according to Rat NP_001015024.2). Recommended concentration: 0.03=0.3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC