Goat Anti-Pleckstrin Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06169
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FLJ27168|p47|P47|pleckstrin|PLEK
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002655.2
Blocking Peptide
EBP06169
Immunogen
Peptide with sequence C-EWIKAIQMASRTGK, from the C Terminus of the protein sequence according to NP_002655.2.
Peptide Sequence
C-EWIKAIQMASRTGK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
5341
Product Grade
IHC Results
Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 40-45kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1kDa according to Human NP_002655.2). Recommended concentration: 0.01-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 ( hepatocellular carcinoma) cell lysate.
Application Type
Pep-ELISA, WB, IF, FC, IHC

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