Goat Anti-PHLDA2 / IPL Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

tumor-supressing STF cDNA 3|tumor suppressing subtransferable candidate 3|tumor suppressing subchromosomal transferable fragment cDNA 3|pleckstrin homology-like domain family A member 2|p17-Beckwith-Wiedemann region 1C|imprinted in placenta and liver|tumor-supressing STF cDNA 3|tumor suppressing subtransferable candidate 3|PHLDA2|HLDA2|BWR1C|BRW1C|IPL|TSSC3|pleckstrin homology-like domain, family A, member 2|PHLDA2

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

NP_003302.1

EBP06184

Peptide with sequence C-EPSRPSPQPKPRTP, from the C Terminus of the protein sequence according to NP_003302.1.

C-EPSRPSPQPKPRTP

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

7262

Paraffin embedded Human Placenta and Prostate. Recommended concentration: 3.75µg/ml.

Antibody detection limit dilution 1:32000.

Approx. 22kDa band observed in lysates of cell line MDA-MB-231 and in preliminary testing of MCF7 cell lysate (calculated MW of 17.1kDa according to NP_003302.1). This molecular weight has been observed by other sources. Recommended concentration 2-3µg/ml Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF, IHC, FC