Goat Anti-SPA1 / SIPA1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

SIPA1|SPA1|MGC17037|signal-induced proliferation-associated gene 1|p130 SPA-1|GTPase-activating protein Spa-1|signal-induced proliferation-associated 1|MGC102688|signal-induced proliferation-associated gene 1|signal-induced proliferation-associated protein 1

NP_694985.28; NP_006738.3

EBP06260

Peptide with sequence C-ASKQLGSPTADLA, from the C Terminus of the protein sequence according to NP_694985.28; NP_006738.3.

Both variants represent identical protein (NP_694985.28 and NP_006738.3).

C-ASKQLGSPTADLA

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

6494

Antibody detection limit dilution 1:32000.

Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272(44):28081-8; PMID: 9346962). Recommended concentration: 1-3µg/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.

Pep-ELISA, WB