Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names transgelin variant 2|DKFZp686B01212|TAGLN1|DKFZp686P11128|SM22-alpha|transgelin|WS3-10|SMCC|SM22|TAGLN | |
Accession ID NP_003177.2 | |
Blocking Peptide EBP06281 | |
Immunogen Peptide with sequence C-MTGYGRPRQIIS, from the C Terminus of the protein sequence according to NP_003177.2. | |
Product Comments NP_003177.2 and NP_001001522.1 represent identical protein.
The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumour suppressor. | |
Peptide Sequence C-MTGYGRPRQIIS | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 6876 | |
Mouse Gene ID 21345 | |
Rat Gene ID 25123 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 24kDa band observed in Human Skeletal Muscle and Testis lysates, and approx 25kDa in Mouse Skeletal Muscle lysates (calculated MW of 22.6kDa according to Human NP_003177.2 and Mouse NP_035656.1). These molecular weights ares routinely observed by other sources. Recommended concentration: 0.003-0.03 µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB |
Goat Anti-SM22 Alpha / Transgelin Antibody
$423.00
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Selected References [{"pmid": 25457224, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "CTGF/CCN2 exerts profibrotic action in myoblasts via the up-regulation of sphingosine kinase-1/S1P3 signaling axis: Implications in the action mechanism of TGF?.", "author": "Bruno G, Cencetti F, Pertici I, Japtok L, Bernacchioni C, Donati C, Bruni P.", "journal": "Biochim Biophys Acta. 2015 Feb;1851(2):194-202."}, {"pmid": 32907751, "intro": "This antibody (previous batch) has been successfully used in Western blot on Human:", "title": "Sphingosine 1-phosphate receptors are dysregulated in endometriosis: possible implication in transforming growth factor ?-induced fibrosis", "author": "Caterina Bernacchioni, Tommaso Capezzuoli, Valentina Vannuzzi, Francesca Malentacchi, Francesca Castiglione, Francesca Cencetti, Marcello Ceccaroni, Chiara Donati, Paola Bruni, Felice Petraglia", "journal": "Fertil Steril. 2020 Sep 6;S0015-0282(20)30766-4."}, {"pmid": 19337690, "intro": "This antibody (previous batch) has been successfully used in Western blot on Human:", "title": "Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells.", "author": "Nincheri P, Luciani P, Squecco R, Donati C, Bernacchioni C, Borgognoni L, Luciani G, Benvenuti S, Francini F, Bruni P.", "journal": "Cell Mol Life Sci. 2009 May;66(10):1741-54."}, {"pmid": 20089836, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis.", "author": "Cencetti F, Bernacchioni C, Nincheri P, Donati C, Bruni P.", "journal": "Mol Biol Cell. 2010 Mar 15;21(6):1111-24."}, {"pmid": 21629665, "intro": "This antibody (previous batch) has been successfully used in Western blot on Human:", "title": "Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for\r\nGATA6.", "author": "Donati C, Marseglia G, Magi A, Serratì S, Cencetti F, Bernacchioni C, Nannetti G, Benelli M, Brunelli S, Torricelli F, Cossu G, Bruni P.", "journal": "PLoS One. 2011;6(5):e20389."}, {"pmid": 38072366, "intro": "This antibody (previous batch) has been successfully used in WB on Human:", "title": "Sphingosine-1-phosphate receptor 3 is a non-hormonal target to counteract endometriosis-associated fibrosis", "author": "Caterina Bernacchioni, Margherita Rossi, Valentina Vannuzzi, Matteo Prisinzano, Isabelle Seidita, Maryam Raeispour, Angela Muccilli, Francesca Castiglione, Paola Bruni, Felice Petraglia, Chiara Donati", "journal": "Fertil Steril. 2024 Apr;121(4):631-641."}, {"pmid": 34445567, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "Antagonizing S1P3 Receptor with Cell-Penetrating Pepducins in Skeletal Muscle Fibrosis", "author": "Angela Corvino et al.", "journal": "Int J Mol Sci. 2021 Aug; 22(16): 8861."}] |