Goat Anti-ECSM2 Antibody

$464.00

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SKU: EB06374
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
hypothetical protein LOC641700|endothelial cell-specific marker 2, endothelial cell-specific molecule 2
Usage Summary
Immunofluorescence: This product has been successfully used in IF on Human: Matsuo K et al, J Biol Chem. 2015 Feb 6;290(6):3784. PMID: 25533470.
Accession ID
NP_001071161.1
Blocking Peptide
EBP06374
Immunogen
Peptide with sequence C-NNGKQSLSAEKVL, from the C Terminus of the protein sequence according to NP_001071161.1.
Peptide Sequence
C-NNGKQSLSAEKVL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Product Grade
IHC Results
Paraffin embedded Human Vessel. Recommended concentration: 5µg/ml. This product has been successfully used in IHC on Human: Matsuo K et al, J Biol Chem. 2015 Feb 6;290(6):3784. PMID: 25533470.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 50kDa band observed in Human Lung and Human Umbilical Cord lysates (calculated MW of 21.3kDa according to NP_001071161.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ref PMID: 18556573). Recommended concentration: 1-3µg/ml.
Application Type
Pep-ELISA, WB, IHC, IF
Selected References
[{"pmid": 25533470, "intro": "This antibody has been successfully used in IHC and IF on Human:", "title": "Loss of apoptosis regulator through modulating IAP expression (ARIA) protects blood vessels fromatherosclerosis.", "author": "Matsuo K, Akakabe Y, Kitamura Y, Shimoda Y, Ono K, Ueyama T, Matoba S, Yamada H, Hatakeyama K, Asada Y, Emoto N, Ikeda K.", "journal": "J Biol Chem. 2015 Feb 6;290(6):3784"}]